Time Course Study of Protein, Metabolite and Lipid Profiles in Type 2 Diabetes Mellitus Rats upon High Fat Diet Intake


School of Pharmaceutical Sciences, USM
Prof. Dr. Gam Lay Harn (PI)
Dr. Baharudin Ibrahim
Assoc. Prof. Dr. Vikneswaran Murugaiyah

Analytical Biochemistry Research Centre, USM
Dr. Mohd Nazri Ismail

Dr. Peter Greimel



As people age, they become more susceptible to disease and disability. Type 2 diabetes (T2DM) is one of the common diseases amongst the aged population, it happened when the body cannot response efficiently to the intake of sugar.  Increased sugar  intake has been associated with the increased prevalence of diabetes. As we observed the eating habits of a group of diabetic patients, we found that although many of the patients was able to have a tight control on their diet, many more do not abide to the suggested diet for diabetic patients. In this study, we aimed to evaluate the time course changes of proteins, lipids and metabolites profiles upon increased intake of high calories food for a prolong period of time in Type 2 diabetic rats model.  The rats will be divided into 7 different groups as 1) Normal Rat with normal chow; 2) Normal Rat with High Fats Diet; 3)Diabetic Rat normal chow; 4) Diabetic Rat normal chow with Metformin; 5) Diabetic Rat with High Fats Diet; 6) Diabetic Rat with High Fats Diet with Metformin; 7) Diabetic Rat with High Fats Diet with MSG.  The proteomics, lipidomics, metabolomics by using LC/MS/MS will be conducted on the urine, feces, blood and different organs of the rats. The data obtained will be useful for the intervention and management of diabetes control.


Foot of an elderly diabetic patient